Adrian Stacy The focus of this treehouse will be upon two of the most studied species in the phylum Deinococcus-Thermus:
Thermus aquaticus Save Thermus aquaticus is a species of bacteria that can tolerate high temperatures, one of several thermophilic bacteria that belong to the Deinococcus—Thermus group.
It is the source of the heat-resistant enzyme Taq DNA polymeraseone of the most important enzymes in molecular biology because of its use in the polymerase chain reaction PCR DNA amplification technique. InThomas D. Brock and Hudson Freeze of Indiana University reported a new species of thermophilic bacteria which they named Thermus aquaticus.
This bacterium is a chemotroph —it performs chemosynthesis to obtain food. However, since its range of temperature overlaps somewhat with that of the photosynthetic cyanobacteria that share its ideal environment, it is sometimes found living jointly with its neighbors, obtaining energy for growth from their photosynthesis.
Morphology Thermus aquaticus is generally of cylindrical shape with a diameter of 0. The rod-shaped bacteria have a tendency to aggregate. Aldolase Studies of this extreme thermophilic bacterium that could be grown in cell culture was initially centered on attempts to understand how protein enzymes which normally inactivate at high temperature can function at high temperature in thermophiles.
InFreeze and Brock published an article describing a thermostable aldolase enzyme from T. Taq I restriction enzyme Most molecular biologists probably became aware of T.
Later, Kary Mullis and other investigators at Cetus Corporation discovered this enzyme could be used in the polymerase chain reaction PCR process for amplifying short segments of DNA , eliminating the need to add enzyme after every cycle of thermal denaturation of the DNA.
The enzyme was also clonedsequencedmodified to produce the shorter 'Stoffel fragment'and produced in large quantities for commercial sale. Other enzymes The high optimum temperature for T.
Other enzymes isolated from this organism include DNA ligasealkaline phosphataseNADH oxidaseisocitrate dehydrogenaseamylomaltaseand fructose 1,6-disphosphate-dependent L-lactate dehydrogenase.
Controversy The commercial use of enzymes from T. Brock's studies, samples of the organism were deposited in the American Type Culture Collectiona public repository.
Other scientists, including those at Cetus, obtained it from there. As the commercial potential of Taq polymerase became apparent in the s, the National Park Service labeled its use as the "Great Taq Rip-off".This thriving bacterium was named Thermus aquaticus (T.
aquaticus). Several enzymes have been isolated from T. aquaticus, the most known of which is Taq DNA polymerase (Taq). Taq can be isolated either from its original source or from its cloned gene expressed in E.
coli. Thermus aquaticus Y51MC23 is one of a number of novel thermophilic species isolated from 88°C water in the northern outflow channel of Bath hot spring in the Lower Geyser Basin of .
such species is the bacterium Thermus aquaticus, found in the hot springs of Yellowstone. From this organism was isolated Taq polymerase, a heat-resistant enzyme crucial for a DNA-amplification technique widely used in research and medical diagnostics (see polymerase chain reaction).
Thermus aquaticus is a species of bacterium that can live in high temperatures, ths species is one of thermophilic bacteria that belong to the Deinococcus-Thermus group.
Thermus aquaticus appears as either a rod or a short slender thread, and the rod-shaped cells will tend to form either a rose shape or a straight line pattern.
Species: Thermus aquaticus – Thermus caldophilus – Thermus thermophilus – Thermus sp. – Thermus filiformis – Thermus sp.
Rt41A – Thermus scotoductus – Thermus sp. Tok6A1 – Thermus sp. SM32 – Thermus brockianus – Thermus oshimai –. Thermus Aquaticus. In any case, one of the most famous strains of thermophilic bacteria actually belongs to a species known as Thermus aquaticus, not Strain